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- Clone
- W19134A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HBRM, BRM, Probable Global Transcription Activator SNF2L2, ATP-Dependent Helicase SMARCA2, BRG1-Associated Factor 190B, Protein Brahma hom*olog, Brahma hom*olog, SNF2-Alpha, BAF190B, BAF190, SNF2L2, SNF2LA, HSNF2a, Sth1p, SNF2, SW12
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
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- publications
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| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 941501 | 25 µg | £81 | ||||
| 941502 | 100 µg | £201 | ||||
Description
SMARCA2 (also known as BRM) is a subunit of the SWI/SNF complex that facilitates chromatin remodeling. As a catalytic subunit, SMARCA2 contains an ATPase domain which uses ATP to alter DNA-histone interactions within a nucleosome. SMARCA2 as part of the chromatin remodeling complex regulates the expression of genes involved in cell cycle, metabolism, development and cancer. In most cases of cancer there is either downregulation or deficiency of the BRM gene. SMARCA2 serves as a target for anticancer therapy. Mutations in SMARCA2 can result in Nicolaides-Baraitser syndrome.
Product Details
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human SMARCA2 peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
- WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25Â- 1.0µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. Neither method was compatible with SMARCA2 staining.
This clone failed to enrich SMARCA2 from HeLa extracts when tested for IP.
- RRID
- AB_2890859 (BioLegend Cat. No. 941501)
AB_2890859 (BioLegend Cat. No. 941502)
Antigen Details
- Structure
- SMARCA2 is a 1,590 amino acid protein with a predicted molecular weight of 181 kD
- Distribution
-
Ubiquitously expressed/Nucleus
- Function
- Chromatin Remodeling
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics
- Antigen References
-
1. Boris G. et al., 2014.Molecular and Cellular Biology.34(6) 1136-1144
2. Euskirchen G. et al., 2012.J Biol Chem.287(27) 30897-905
3. Van Houdt, J.et al., 2012.Nat Genet.44 445-449
4. Vangamudi B. et al., 2015.Cancer Res.75(18) 3865-3878 - Gene ID
- 6595 View all products for this Gene ID
- UniProt
- View information about SMARCA2 on UniProt.org
Documentation
- Technical Data Sheet (pdf)
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Protocols
- Western Blotting Protocol
Related Products
| Description | Clone | Applications |
|---|
Pages
- Quality Control
Related FAQs
There are no FAQs for this product.
Other Formats
View All SMARCA2 Reagents Request Custom Conjugation
| Description | Clone | Applications |
|---|---|---|
| Purified anti-SMARCA2 (BRM) Antibody | W19134A | WB |
Certificate of Analysis
For Research Use Only. Not for diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-SMARCA2 (BRM) Antibody
Whole cell extracts (15 µg total protein) from the indicated...
